Gianpaolo Papaccio

Gianpaolo Papaccio was born in Napoli (Italy) in 1955. He obtained his BA in Medicine and Surgery cum laude in 1979 at the Federico II University of Naples and his Ph.D. in Human Biostructure at the Washington University of Seattle (Seattle, WA, USA) in 1982.

Gianpaolo Papaccio

Gianpaolo Papaccio in 2022
Born	February 13th, 1955 Napoli, Italy
Nationality	Italian
Education	University of Naples “Federico II” (Italy) Washington University -Seattle (USA)
Fields	Medicine and Surgery
Institutions	University of Campania “L. Vanvitelli” Medicine and Surgery School-Naples (IT)
Notes
Top Italian Scientist in Biomedical Sciences

Positions

He is currently Full Professor of Human Histology and Embryology, at the Department of Experimental Medicine, University of Campania “L. Vanvitelli” Naples. He is a member of the Ph.D. Council of Biotechnology at the same University.

Education and career

Gianpaolo Papaccio graduated in Medicine and Surgery from the University Naples “Federico II” in 1979. He got a Ph.D. from the University of Washington (Seattle-USA) with a research project on the 3D reconstruction of pancreatic islets from healthy and type 1 diabetic subjects. He got a position as a Researcher in 1984 in the University of Naples “Federico II”. In 1994 he was appointed as associate professor at the same University and in 2005 he become Full Professor of Human Histology and Embryology at the University of Campania “L. Vanvitelli”.

At the same time he was also physician in the Clinical Hospital of the University as assistant, then Head of the Hospital Division of “Citometry and NGS” for Oncological patients.

Over the years, he has held numerous teaching courses in the said Universities, becoming, since 2013 President of the Degree Course in Medicine and Surgery in English for foreigner and Italian students. This Position was maintained up to 2019.

Research Interests

Gianpaolo Papaccio’s research interests include: Human mesenchymal stem cell (hMSC) biology from isolation to characterization and differentiation into osteogenic, angiogenic and adipogenic lineages. Production in vitro and in vitro of Human bone starting from hMSCs, studies of stem cell adhesion and differentiation to several scaffolds for bone tissue engineering, relationship between hMSCs and cancer stem cells (CSCs) in the tumor bulk of human osteosarcomas and breast cancers and peritumoral tissue, as well as mitochondrial transfer between hMSCs and CSCs.

Research

Studies on Human Mesenchymal Stem Cells

Gianpaolo Papaccio’s studies on Human Mesenchymal stem cells include stem cells from adipose Stromal-Vascular area of Human adipose tissue, taken from patients undergoing plastic surgery or from dental pulps of patients undergoing 3rd molar surgery. Those studies were performed in his laboratory and in collaboration with other research Labs. The studies were the first ones demonstrating that Dental Pulp Stem Cells (DPSCs) were capable to give rise to woven bone in in vitro culture and then, when grafted to patient’s mandible, able to obtain a compact bone.

Studies on Osteogenic, angiogenic and adipogenic Differentiation

Gianpaolo Papaccio’s studies on hMSCs differentiation were focused at differentiating, those stem cells toward bone, vessels and adipose tissue. The studies were performed in 2D and 3D cultures and either bone and vessels as well as adipose tissues were obtained.

Studies on Bone growth in vitro and in vivo (cell grafting)

Gianpaolo Papaccio’s main studies were the first capable to demonstrate that Dental Pulp Stem Cells (DPSCs), that are hMSCs very proliferative, were capable to give rise to woven bone after in vitro culture and then, when grafted into patient’s mandible, were able to give rise to a compact bone in all patients. Other studies demonstrated a pivotal role of HDAC.

Studies on MSCs and CSCs interplay

More recently Gianpaolo Papaccio’s studies were devoted to understand a possible role of hMSCs in cancer biology. The question was: do the hMSCs exert possible effects in modulating the behavior and metabolism of cancer cells? The first studies. have demonstrated that cancer cells allow hMSCs to become endothelial cells stimulating angiogenesis. Further studies have shown that a considerably interplay exists between hMSCs and cancer cells mainly in the peritumoral area with mitochondrial transfer from hMSCs to cancer cells.

Methods

In his studies, he employed several methods of cellular and molecular biology, including: cell isolation and characterization, Cell sorting (by Flux Cytometry), qRT-PCR, WB, ELISA reader, Crispr-Cas9, viral vectors, gene editing, confocal microscopy, immunofluorescence, among others.

Selected Publications

• Papaccio G, Pisanti F.A. and Frascatore S., Acetyl- homocysteine thiolactone-induced increase of Superoxide Dismutase counteracts the effect of subdiabetogenic doses of streptozocin ,Diabetes, 35, 470-474, 1986.

• Papaccio G, Prevention of low-dose-streptozocin induced diabetes by acetyl-homocysteine-thiolactone, Diabetes Res and Clin Practice, 13, 95-102, 1991.

• Papaccio G. and Latronico M., Diabetes incidence and histopathological lesions in animal models, Diabetes Res. and Clin. Practice, 18, 137, 1992.

• Papaccio G., Gangliosides prevent insulitis but not islet B cell desruption in LDS treated mice, Diabetes Res. and Clin. Practice, 19, 9-15, 1993.

• Papaccio G., Chieffi Baccari G., Early insulitis and islet vascular system, Diabetologia, 36, 682, 1993.

• Papaccio G., Nicoletti F, Pisanti F.A., Bendtzen K and Galdieri M, Prevention of spontaneous autoimmune diabetes in NOD mice by transferring in vitro antigen-pulsed syngeneic dendritic cells, “Endocrinology” 141: 1500-1505, 2000.

• Gregorio Laino, Riccardo d’Aquino, Antonio Graziano, Vladimiro Lanza, Francesco Carinci, Giuseppe Pirozzi, Fabio Naro and Gianpaolo Papaccio, Dental pulp stem cells can be detected in aged humans: an useful source for living autologous fibrous bone tissue (LAB). Journal of Bones and Mineral Research. Res,20:1394-402. 2005.

• d'Aquino R, Graziano A, Sampaolesi M, Laino G, Pirozzi G, De Rosa A, Papaccio G., Human postnatal dental pulp cells co-differentiate into osteoblasts and endotheliocytes: a pivotal synergy leading to adult bone tissue formation. Cell Death & Differentiation, 14(6):1162-1171, 2007

• A. Graziano, R. d’Aquino, G. Laino, A. Proto, M. T. Giuliano, G. Pirozzi, A. De Rosa, D. Di Napoli and G. Papaccio. Human CD34+ stem cells produce bone nodules in vivo. Cell Proliferation, 2008, 41, 1-11

• L. Spath, V. Rotilio, M. Alessandrini, G. Gambara, L. De Angelis, M. Mancini, T. A. Mitsiadis, E. Vivarelli, F. Naro, A. Filippini, G. Papaccio, Explant-derived human dental stem pulp stem cells enhance differentiation and proliferation potentials, Journal of Cellular and Molecular Medicine, 2010; 14:1635-44

• Mangano C, De Rosa A, Desiderio V, d’Aquino R, Piattelli A, De Francesco F, Tirino V, Mangano F and Papaccio G, The Osteoblastic Differentiation of Dental Pulp Stem Cells and Bone Formation on Different Titanium Surface Textures, Biomaterials, 31: 3543–3551, 2010

• Virginia Tirino, Francesca Paino & Riccardo d’Aquino, Vincenzo Desiderio, Alfredo De Rosa, and Gianpaolo Papaccio. Methods for the identification, characterization and banking of human dpscs: current strategies and perspectives, Stem Cell Reviews and Reports, 7: 608-15, 2011

• Tirino V, Desiderio V, Paino F, De Rosa A, Papaccio F, Fazioli F, Pirozzi G and Papaccio G, Human primary bone sarcomas contain cd133+ cancer stem cells displaying high tumourigenicity in vivo, FASEB J, 25: 2022-30, 2011

• Tirino V, Desiderio V, Paino F, De Rosa A, Papaccio F, La Noce M, Laino L, De Francesco F, Papaccio G., Cancer stem cells in solid tumors: an overview and new approaches for their isolation and characterization, FASEB J, 27,13-24, 2013.

10) Paino F, La Noce M, Tirino V, Naddeo P, Desiderio V, Pirozzi G, De Rosa A, Laino L, Altucci L, Papaccio G. Histone Deacetylase inhibition with Valproic Acid down-regulates Osteocalcin gene expression in Human Dental Pulp Stem Cells and Osteoblasts: Evidence for HDAC2 involvement, “Stem Cells”, 32, 279-89, 2014.

• Paino F, La Noce M, Giuliani A, De Rosa A, Mazzoni S, Laino L, Amler E, Papaccio G, Desiderio V, Tirino V., Human DPSCs fabricate vascularized woven bone tissue: a new tool in bone tissue engineering. Clinical Science. 2017 Apr 25;131(8):699-713.Epub 2017 Feb 16.

• Papaccio F, Paino F, Regad T, Papaccio G, Desiderio V, Tirino V. Concise Review: Cancer Cells, Cancer Stem Cells, and Mesenchymal Stem Cells: Influence in Cancer Development. Stem Cells Translational Medicine. 2017 Dec;6(12):2115-2125. Epub 2017 Oct 26.

• Paino F, La Noce M, Tirino V, Naddeo P, Desiderio V, Pirozzi G, De Rosa A, Laino L, Altucci L, Papaccio G. Histone Deacetylase inhibition with Valproic Acid down-regulates Osteocalcin gene expression in Human Dental Pulp Stem Cells and Osteoblasts: Evidence for HDAC2 involvement. Stem Cells. 2013 Sep 16. 2014 Jan;32(1):279-89

• Paino F, La Noce M, Di Nucci D, Nicoletti GF, Salzillo R, De Rosa A, Ferraro GA, Papaccio G, Desiderio V, Tirino V. Human adipose stem cell differentiation is highly affected by cancer cells both in vitro and in vivo: implication for autologous fat grafting. Cell Death & Disease, 2017 Jan 19;8(1).

• Paino F, La Noce M, Di Nucci D, Nicoletti GF, Salzillo R, De Rosa A, Ferraro GA, Papaccio G, Desiderio V, Tirino V. Human adipose stem cell differentiation is highly affected by cancer cells both in vitro and in vivo: implication for autologous fat grafting, Cell Death. Dis., 19, e2568, 2017.

• Papaccio F, Paino F, Regad T, Papaccio G, Desiderio V, Tirino V. Concise Review: Cancer Cells, Cancer Stem Cells, and Mesenchymal Stem Cells: Influence in Cancer Development., Stem Cells Transl Med., 6, 2115-2125, 2017.

• Mele L, Paino F, Papaccio F, Regad T, Boocock D, Stiuso P, Lombardi A, Liccardo D, Aquino G, Barbieri A, Arra C, Coveney C, La Noce M, Papaccio G, Caraglia M, Tirino V, Desiderio V. A new inhibitor of glucose-6-phosphate dehydrogenase blocks pentose phosphate pathway and suppresses malignant proliferation and metastasis in vivo, Cell Death Dis., 9, 572, 2018

• La Noce M, Paino F, Mele L, Papaccio G, Regad T, Lombardi A, Papaccio F, Desiderio V, Tirino V, HDAC2 depletion promotes osteosarcoma's stemness both in vitro and in vivo: a study on a putative new target for CSCs directed therapy, J Exp Clin Cancer Res, 37, 296, 2018.

• Martini G, Cardone C, Vitiello PP, Belli V, Napolitano S, Troiani T, Ciardiello D, Della Corte CM, Morgillo F, Matrone N, Sforza V, Papaccio G, Desiderio V, Paul MC, Moreno-Viedma V, Normanno N, Rachiglio AM, Tirino V, Maiello E, Latiano TP, Rizzi D, Signoriello G, Sibilia M, Ciardiello F, Martinelli E, [EPHA2 Is a Predictive Biomarker of Resistance and a Potential Therapeutic Target for Improving Antiepidermal Growth Factor Receptor Therapy in Colorectal Cancer], Mol Cancer Ther, 18,845-855, 2019

• La Noce M, Mele L, Laino L, Iolascon G, Pieretti G, Papaccio G, Desiderio V, Tirino V, Paino F, Cytoplasmic Interactions between the Glucocorticoid Receptor and HDAC2 Regulate Osteocalcin Expression in VPA-Treated MSCs, Cells, 8, 217, 2019

• Mele L, la Noce M, Paino F, Regad T, Wagner S, Liccardo D, Papaccio G, Lombardi A, Caraglia M, Tirino V, Desiderio V, Papaccio F. Glucose-6-phosphate dehydrogenase blockade potentiates tyrosine kinase inhibitor effect on breast cancer cells through autophagy perturbation, J Exp Clin Cancer Res., 38, 160, 2019

• Mele L, Del Vecchio V, Liccardo D, Prisco C, Schwerdtfeger M, Robinson N, Desiderio V, Tirino V, Papaccio G, La Noce M, The role of autophagy in resistance to targeted therapies, Cancer Treat Rev., 88:102043, 2020.

• Mele L, Del Vecchio V, Marampon F, Regad T, Wagner S, Mosca L, Bimonte S, Giudice A, Liccardo D, Prisco C, Schwerdtfeger M, La Noce M, Tirino V, Caraglia M, Papaccio G, Desiderio V, Barbieri A. β 2-AR blockade potentiates MEK1/2 inhibitor effect on HNSCC by regulating the Nrf2-mediated defense mechanism, Cell Death Dis, 11, 850, 2020.

• Vitiello PP, Martini G, Mele L, Giunta EF, De Falco V, Ciardiello D, Belli V, Cardone C, Matrone N, Poliero L, Tirino V, Napolitano S, Della Corte C, Selvaggi F, Papaccio G, Troiani T, Morgillo F, Desiderio V, Ciardiello F, Martinelli E., 33407715/ Vulnerability to low-dose combination of irinotecan and niraparib in ATM-mutated colorectal cancer., Journal of Experimental and Clinical Cancer Research, 40, 15, 2021.

• La Gatta A, Tirino V, Cammarota M, La Noce M, Stellavato A, Pirozzi AVA, Portaccio M, Diano N, Laino L, Papaccio G, Schiraldi C. Gelatin-biofermentative unsulfated glycosaminoglycans semi-interpenetrating hydrogels via microbial-transglutaminase crosslinking enhance osteogenic potential of dental pulp stem cells, Regen Biomater, 8, rbaa 052, 2021.

• La Noce M, Stellavato A, Vassallo V, Cammarota M, Laino L, Desiderio V, Del Vecchio V, Nicoletti GF, Tirino V, Papaccio G, Schiraldi C and Ferraro GA., Hyaluronan-Based Gel Promotes Human Dental Pulp Stem Cells Bone Differentiation by Activating YAP/TAZ Pathway, Cells, 10, 2899, 2021.

External links

• Gianpaolo Papaccio - Università degli Studi della Campania "Luigi Vanvitelli"
• Gianpaolo Papaccio - Google Scholar